Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-09-01
1996-05-07
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 243, 536 231, 935 6, 935 10, 935 78, C12Q 168, C07H 2104
Patent
active
055145469
ABSTRACT:
The present invention provides stem-loop oligonucleotides containing a double-stranded stem domain of at least about 2 base pairs and a single-stranded loop domain. The loop domains of the present oligonucleotides include at least one parallel binding (P) domain separated by at least about 3 nucleotides from a corresponding anti-parallel binding (AP) domain. Each P and corresponding AP domain of the present oligonucleotides can bind detectably to one strand of a defined nucleic acid target wherein the P domain binds in a parallel manner to the target and the corresponding AP domain binds in an anti-parallel manner to the target. The present stem-loop oligonucleotides can bind to both single-stranded and double-stranded target nucleic acids. The present invention also provides methods of using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.
REFERENCES:
patent: 4868103 (1989-09-01), Stavrianopoulos
patent: 5422251 (1995-06-01), Fresco
Baumann et al. (1988) Interaction of DNA Hairpin Loops and a Complementary Strand by a Triplet of Base Pairs. Biochem. Biophys. Res. Commun. 157:986-991.
D'Souza et al. (1992) Strong Binding of Single-Stranded DNA by Stem-Loop Oligonucleotides. J. Biomolec. Struc. & Dyam. 10:141-152.
Durand et al. (1992) Triple-Helix Formation by an Oligonucleotide Containing One (dA).sub.12 and Two (dT).sub.12 Sequences Bridged by Two Hexaethylene Glycol Chains. Biochemistry 31:9197-9204.
Ecker et al. (1992) Pseudo-Half-Knot Formation with RNA. Science 257:958-961.
Kool (1991) Molecular Recognition by Circular Oligonucleotides: Increasing the Selectivity of DNA Binding. J. Amer. Chem. Soc. 113:6265-6266.
Giovannangeli et al. (1991) Single-Stranded DNA as a Target for Triple-Helix Formation. J. Amer. Chem. Soc. 113:7775-7777.
Manzini et al. (1990) Triple Helix Formation by Oligopurine-Oligopyrimidine DNA Fragments. J. Mol. Biol. 213:833-843.
Prakash et al. (1992) Structural Effects in the Recognition of DNA by Circular Oligonucleotides. J. Amer. Chem. Soc. 114:3523-3527.
Prakash et al. (Apr. 5-10, 1992) Recognition of DNA and RNA by Circular Oligonucleotides: Strong Binding and High Selectivity. American Chemical Society 203rd National Meeting, abstract ORGN473.
Prakash et al. (Apr. 5-10, 1992) Structural Effects in the Recognition of Single-Stranded DNA by Circular Oligonucleotides. American Chemical Society 203rd National Meeting, abstract ORGN341.
Prakash et al. (1991) Molecular Recognition by Circular Oligonucleotides: Strong Binding of Single-Stranded DNA and RNA. J. Chem. Soc. Chem. Commun. 113:1161-1163.
Rumney IV et al. (Aug. 23, 1992) DNA Recognition by Hybrid Oligoether-Oligonucleotide Macrocycles. American Chemical Society 204th National Meeting, abstract ORGN394.
Voloshin et al. (1988) Chemical Probing of Homopurine-Homopyrimidine Mirror Repeats in Supercoiled DNA. Nature 333:475-476.
Xodo et al. (1990) Spectroscopic and Calorimetric Investigation on the DNA Triplex Formed by d(CTCTTCTTTCTTTTCTTTCTTCTC) and d(GAGAAGAAAGA) at acidic pH. Nucleic Acids Res. 18:3557-3564.
D'Souza et al., J. Biomolec Structure & Dynamics (Sep. 2, 1992) 10:141-152.
Jones W. Gary
Myers Carla
Research Corporation Technologies Inc.
LandOfFree
Stem-loop oligonucleotides containing parallel and antiparallel does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Stem-loop oligonucleotides containing parallel and antiparallel , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stem-loop oligonucleotides containing parallel and antiparallel will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1226442