Method for characterizing single cells based on RNA amplificatio

Details Method for characterizing single cells based on RNA amplificatio Method for characterizing single cells based on RNA amplificatio

1993-07-26

1996-05-07

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 15, 435 911, 435 9121, 435 912, 435 915, 435 9151, C12Q 168, C12P 1934

Patent

active

055145450

ABSTRACT:
A method is provided for characterizing cells at the molecular level by amplifying RNA from selected single cells by microinjecting primer, nucleotides and enzyme into acutely dissociated cells to produce amplified antisense RNA; reamplifying the amplified antisense RNA (aRNA) produced by using random hexanucleotides to prime cDNA synthesis from aRNA, and then detecting messages in the amplified RNA. The invention is useful for characterization of cell identity or physiological state.

REFERENCES:
patent: 5021335 (1991-06-01), Tecott et al.
patent: 5168038 (1992-12-01), Tecott et al.
Eberwine et al., PNAS 89, 3010-3014, 1992.
Sambrook et al., "Molecular Cloning", Cold Spring Harbor Laboratory Press, 1989.
Eberwine et al., in "Methods of Enzymology", Wu ed., vol. 216, pp. 80-100, 1992.
Van Gelder et al., PNAS 87, 1663-1667, 1990.
Chang et al., Biochem. Biophys. Res. Comm. 157(2), 698-704, 1988.
Liu et al., J. Virol. Methods 35, 227-236, 1991.

Affiliated with

Also associated with

Rating

Method for characterizing single cells based on RNA amplificatio does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for characterizing single cells based on RNA amplificatio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for characterizing single cells based on RNA amplificatio will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1226438