Synthesis of 7-deaza-2'deoxyguanosine nucleotides

Details Synthesis of 7-deaza-2'deoxyguanosine nucleotides Synthesis of 7-deaza-2'deoxyguanosine nucleotides

1997-05-16

1999-11-23

Crane, L. Eric

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2626, 536 2671, 536 2672, 536 2713, 536 231, 536 243, 536 2781, 435 911, C07H 1914, C07H 1220

Patent

active

059903031

ABSTRACT:
The present invention provides 7-deaza-2'-deoxy-guanosine nucleotides of the general formula: ##STR1## wherein R is a --PO.sub.3 H.sub.2, --P.sub.2 O.sub.6 H.sub.3 or --P.sub.3 O.sub.9 H.sub.4 residue or an alkali metal, alkaline earth metal or ammonium salt of the phosphate groups.
The present invention also provides processes for the preparation of these nucleotides and is also concerned with the use thereof in the sequencing of DNA.

REFERENCES:
patent: 3337530 (1967-08-01), Hanze
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4804748 (1989-02-01), Seela
patent: 5091310 (1992-02-01), Innis
patent: 5142033 (1992-08-01), Innis
patent: 5480980 (1996-01-01), Seela
Winkeler et al., "Synthesis of 2-Amino-7-(2'-deoxy-.beta.-D-erythro-pentofuranosyl)-3,7-dihydro-4H-pyrrol o[2,3-d]pyrimidin-4-one, a New Isostere of 2'-Deoxyguanosine," J. Org. Chem., 48(18), 3119-3122 (1983).
Barr et al., "7-Deaza-2'-Deoxyguanosine-5'-Triphosphate: Enhanced Resolution in M13 Dideoxy Sequencing," BioTechniques, 4(5), 428-432 (1986).
Mizusawa et al., "Improvement of the Dideoxy Chain Termination Method of DNA Sequencing by Use of Deoxy-7-deazaguanosine Triphosphate in Place of GTP," Nucleic Acids Research, 14(3), 1319-1324 (1986).
Sanger et al., "DNA Sequencing with Chain-Terminating Inhibitors," Proc. Nat. Acad. Sci. USA, 74(12), 5463-5467 (1977).
Seela et al., "Poly(7-deazaguanylic acid), the Homopolynucleotide of the Parent Nucleoside of Queosine," Biochemistyr, 21(18), 4338-4343 (1982).
Sen et al., "A Sodium-Potassium Switch in the Formation of Four-Stranded G4-DNA," Nature, 344, 410-414 (1990).

Affiliated with

Also associated with

Rating

Synthesis of 7-deaza-2'deoxyguanosine nucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Synthesis of 7-deaza-2'deoxyguanosine nucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthesis of 7-deaza-2'deoxyguanosine nucleotides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1223317