Method of marking biomolecules using horseradish peroxidase

Details Method of marking biomolecules using horseradish peroxidase Method of marking biomolecules using horseradish peroxidase

1998-06-26

1999-11-23

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435188, 435964, 436544, 5303913, 5303915, 530402, G01N 33535

Patent

active

059898429

ABSTRACT:
The invention is a method for the enzymatic labeling of biomolecules, such as immunoglobulin, peptide, hormone, or hapten, which involves oxidizing horseradishperoxidase (HRP) with a periodate, crosslinking the oxidized HRP with an .alpha.,.OMEGA.-diaminoalkane, and coupling the biomolecule with the crosslinked, oxidized HRP.

REFERENCES:
patent: 4655836 (1987-04-01), Mrawert et al.
patent: 4657853 (1987-04-01), Freytag et al.
patent: 5057313 (1991-10-01), Shih et al.
patent: 5534414 (1996-07-01), Pollner et al.
A.J. Pesce et al.: Preparation and Analysis of Peroxidase Antibody and Alkaline Phaophatase Antibody Conjugates, INSERM Symposium No. 2, North Holland Publ., 1976, pp. 7-23.
M.B.Wilson et al.: Recent Developments in the Periodate Method of Conjugating Horseradish Peroxidase to Antibodies, W. Knapp et al. Immunofluorscene & Related Statininf, Elsevier Press 1978, pp. 215-224.
P. K. Nakane et al.: Peroxidase-Labeled Antibody, J. Histochem. Cytochem, 22, 12, 1974, pp. 1084-1090.
K. Fujiwara: New Hapten-Protein Conjugation Method Using N(m-aminobenzyloxy) succinimide, etc., J. Immun. Methods, 175 (1994), Elsevier, pp. 123-129.

Affiliated with

Also associated with

Rating

Method of marking biomolecules using horseradish peroxidase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of marking biomolecules using horseradish peroxidase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of marking biomolecules using horseradish peroxidase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1220331