Elution and identification of T cell epitopes from viable cells

Details Elution and identification of T cell epitopes from viable cells Elution and identification of T cell epitopes from viable cells

1995-06-07

1999-11-23

Feisee, Lila

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Cancer cell or component thereof

514 21, 530344, 435 701, A61K 3400, A61K 3900, C07K 500, C12P 2102

Patent

active

059895659

ABSTRACT:
Methods are provided for eluting peptides that are bound to major histocompatibility complex ("MHC") molecules expressed on the cell surfaces of viable cells that have at least one MHC-peptide complex on the surfaces of the cells, the method comprising incubating the cells in the presence of peptide elution buffer, preferably comprising iso-osmotic, citrate-phosphate buffer at a pH of approximately 3.3, for between about 15 seconds and one minute. Using these methods a naturally processed melanoma peptide recognized by CD8.sup.+ cytotoxic T lymphocytes has been identified.

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