Method for increasing the hydrolytic activity of starch hyrdolas

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435201, 127 36, C12P 1914, C12N 926, C13K 3000

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active

058887814

ABSTRACT:
A method for increasing the catalytic rate of starch hydrolases comprising reacting a starch hydrolase with a substrate therefor in the presence of about 0.001 to 80% (w/v) of an ethylene glycol or polyethylene glycol.

REFERENCES:
patent: 5162210 (1992-11-01), Sierks et al.
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Chen et al. "Lactose hydrolysis by beta-galactosidase in aqueous two-phase systems," J. Ferm. Bioeng. (1991) 71(3): 168-75, 1991.
Larsson et al. "Integration of bioconversion and downstream processing: Starch hydrolysis in an aqueous two-phase system," Biotechnol. Bioeng. (1989) 33: 758-66, 1989.
Delbourg et al. "Effect of PEG and other additives on cyclodextrin production by Bacillus macerans cyclomaltodextrin-glycolsyl-transferase," Biotech. Lett. (1993) 15(2): 157-162, Feb. 1993.
Hayashida et al. "Enhancement of enzymatic production of cyclodextrans by adding polyethylene glycol or polypropylele glycol," J. Fermentation Bioeng. (1992) 7(3): 239-240, 1992.
Semichaevskii et al. "Hydrolysis of soluble starch by anylosubtitin G10x in media containing polyethylene glycol," Appl. Biochem. Microbiol. (1979) 45(2): 122-128, English translation, 1979.
Zaw et al, "Increasing the Catalytic Activity of Glucoamylase", 211th American Chemical Society National Meeting and Exposition Program, pp. viii, 69 and Abstract, New Orleans, LA, (March 27-28, 1996).

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