Screening test for large intestinal cancer

Details Screening test for large intestinal cancer Screening test for large intestinal cancer

1986-07-24

1989-08-15

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 14, 435 25, 435810, 436501, 436518, 436808, 436813, 436827, C12Q 126, C12Q 154, G01N 33543, G01N 33574

Patent

active

048574570

ABSTRACT:
The disaccharide .beta.-D-Gal (1->>3)-D-GalNAc, which specifically binds with peanut agglutinin (PNA) or oxidized by galactose oxidase, has been discovered in the colorectal mucus of patients with cancer or precancer. Because of the presence of .beta.-D-Gal-(1>>3)-D-GalNAc also on neuraminidase treated erythrocytes of the ABO type, their competitive binding with PNA has been exploited to develop a hemagglutination inhibition assay. Additional methods of simple detection of this disaccharide include a latex agglutination test, enzyme-avidin-biotinylated PNA, and a galactose oxidase strip test. This rapid, simple and inexpensive assay is designed to test the presence of .beta.-D-Gal-(1>>3)-D-GalNAc in large intestine mucus obtained by routine digital-rectal examination and has the potential for screening populations for large intestinal carcinomas.

REFERENCES:
patent: 4334017 (1982-06-01), Plotkin et al.
patent: 4571382 (1986-02-01), Adachi
Boland et al., Proc. Nat'l. Acad. Sci. U.S.A., 79, "Alterations In Human Colonic Mucin Occurring With Cellular Differentiation and Malignant Transformation", 2051-2055 (1982).
Hjelm et al., Methods of Enzymatic Analysis, vol. 3, Academic Press, Inc., New York, 1282-1287 (1974).
Lehman et al., Cancer, 53(2): 272-277, "Peanut Lectin Binding Sites In Transitional Cell Carcinoma of the Urinary Bladder" (1984).
L. C. Hoskins & E. T. Boulding, J. Clin. Invest 67: 163-172 (Jan. 1981).

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