Method of compensating and calibrating a flow cytometer, and mic

Details Method of compensating and calibrating a flow cytometer, and mic Method of compensating and calibrating a flow cytometer, and mic

1987-12-04

1989-08-15

Richman, Barry S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436 10, 424 3, G01N 5300, G01N 3100

Patent

active

048574511

ABSTRACT:
A kit of highly uniform microbead standards for flow cytometer alignment, compensation, and calibration, comprising a blank and/or auto-fluorescent microbead population, together with two or more series of calibrated microbead populations which match the fluorescence spectra of labeled samples to be measured on the flow cytometer. Also disclosed is a corresponding method to align, compensate, and calibrate a flow cytometer so as to make measurements on corresponding samples comparable and independent of the specific instrument and instrument settings.

REFERENCES:
patent: 4609689 (1986-09-01), Schwartz et al.
patent: 4694035 (1987-09-01), Kasai et al.
patent: 4698262 (1987-10-01), Schwartz et al.
patent: 4699826 (1987-10-01), Schwartz et al.
patent: 4699828 (1987-10-01), Schwartz et al.
patent: 4714682 (1987-12-01), Schwartz
patent: 4751188 (1988-06-01), Valet

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