Apparatus for conducting electrophoresis experiments

Details Apparatus for conducting electrophoresis experiments Apparatus for conducting electrophoresis experiments

1997-03-28

1999-03-30

Redding, David R.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204456, 204466, 204467, 204616, 204621, G01N 2722

Patent

active

058883690

ABSTRACT:
An apparatus of conducting electrophoresis experiments including a container for receiving an electrophoresis buffer and a buffer core assembly for holding gel-containing cassettes that are immersed in the buffer for molecular separation of an electrophoresis sample. The buffer core assembly defines a flow path for the electrophoresis buffer which can be circulated by means of a heat exchanger and a pump to effect controlled heat exchange between the buffer and the cassette surfaces thereby maintaining the gel and the electrophoresis process at a desirable temperature uniformly over the surfaces of the gel cassette. The buffer core assembly also has upper well for receiving a second electrically chargeable buffer in contact with the gel so as to impose an electric field on the gel for the electrophoresis in conjunction with the first buffer. The well is isolated from the first buffer to prevent mixing of the buffers and to reduce the risk of an electrical short.

REFERENCES:
patent: 5158661 (1992-10-01), Hansen
Hongyo, T., et al., "Cold SSCP': A Simple, Rapid and Non-radioactive Method for Optimized Single-Strand Conformation Polymorphism Analysis", Nucleic Acids Research, vol. 21, No. 16, 3637-3642 (1993).
Yoshimoto, H.I.., et al, "Multiple Fluorescence-Based PCR-SSCP Analysis", Biotechniques, 16, 296-306 (1994).
Instruction Manual for C.B.S. Scientific Company, Inc.,'s Denaturing Gradient Gel Electrophoresis System (1994).
DNA Sequencing Guide, United States Biochemical Corporation, Gel Electrophoresis Section (Date Unknown).
BioTherm Corporation, sales literature for "Cool Gel" Mini Apparatus (1994).
Integrated Separation Systems, sales literature for "Maxi 2-Gel Device" with built-in cooling coil (1994).
Owl Scientific,Inc., sales literature, "Penguin" P8DS-1, P9DS-1, and P10DS-1, Water Cooled Dual Gel System.
Stratagene, sale literature, "StrataTherm Cold Temperature Controller and Feathervolt Power Supply", (1994).
Bio-Rad, sales literature, "Sequi-Gen II Sequencing Cell." (1994).
Hoefer, sales literature, "Mighty Small II Dual Cooled Vertical Slab Unit."
Fotodyne, sales literature, "Buffer Cooler/Circulator and Vertical Cell II Air Cooled Chamber."

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