Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1991-10-29
1994-02-15
Tung, T.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, 2041821, G01N 2726, G01N 27447
Patent
active
052864340
ABSTRACT:
A process for the electrophoretic separation of charged macromolecules includes applying to the macromolecules a periodic sequence of pulses. Each period comprises a multiplicity of electric field pulses of negative and positive polarities. The negative polarity pulses are applied for a longer total time duration than the positive polarity pulses within each period. The average intensity of the negative polarity pulses is less than the average intensity of the positive polarity pulses.
REFERENCES:
patent: 3948743 (1976-04-01), Monthony et al.
patent: 4059501 (1977-11-01), Strickler
patent: 4207166 (1980-06-01), Dahms
patent: 4244513 (1981-01-01), Fayer et al.
patent: 4391688 (1983-07-01), Hamelin
patent: 4433299 (1984-02-01), Kawai et al.
patent: 4441972 (1984-04-01), Pohl
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4541910 (1985-09-01), Daris, III et al.
patent: 4545888 (1985-10-01), Walsh
patent: 4617103 (1986-10-01), Lovegrove
patent: 4631120 (1986-12-01), Pohl
patent: 4632743 (1986-12-01), Yoshikumi et al.
patent: 4695548 (1987-09-01), Cantor et al.
patent: 4729823 (1988-03-01), Guevara, Jr.
patent: 4732656 (1988-03-01), Hurd
patent: 4737251 (1988-04-01), Carle et al.
patent: 4740283 (1988-04-01), Laas et al.
patent: 4747918 (1988-05-01), Wassenberg, II
patent: 4786387 (1988-11-01), Whitlock
patent: 4794075 (1988-12-01), Ford et al.
patent: 4830726 (1989-05-01), Stamato et al.
patent: 5106468 (1992-04-01), Chimenti
C. L. Smith and C. R. Cantor; "Pulsed-Field Gel Electrophoresis of Large DNA Molecules", Nature, vol. 319, pp. 701-702 (1986).
L. M. Corcoran; "Molecular Karyotypes: Separating Chromosomes on Gels"; BioEssays, vol. 3, No. 6, pp. 269-271 (1985).
T. H. N. Ellis, W. G. Cleary, K. W. G. Burcham and B. A. Bowen; "Ramped Field Inversion Gel Electrophoresis: A Cautionary Note"; Nucleic Acids Research, vol. 15, No. 13, 1987, p. 5489.
R. G. Snell and R. J. Wilkins; "Separation of Chromosomal DNA Molecules from C. albicans by `Pulsed Field Gel Electrophoresis"; Nucleic Acids Research, vol. 14, No. 11, pp. 4401-4406 (1986).
T. Jamil and L. S. Lerman; "Dependence of the Electrophoretic Mobility of DNA in Gels on Field Intermittency"; Journal of Biomolecular Structure and Dynamics, vol. 2, No. 5; pp. 963-966 (1985).
"Prediction of Chain Elongation in the Reptation Theory of DNA Gel Electrophoresis"; Biopolymers, vol. 24, No. 12, pp. 2181-2184 (1985).
G. W. Slater and J. Noolandi; "On the Reptation Theory of Gel Electrophoresis", Biopolymers, vol. 25, No. 3, pp. 431-454 (1986).
K. Gardiner, W. Laas, and D. Patterson; "Fractionation of Large Mammalian DNA Restriction Fragments Using Vertical Pulsed--Field Gradient Gel Electrophoresis"; Somatic Cell and Molecular Genetics, vol. 12, No. 2, pp. 185-195 (1986).
D. A. Hardy et al.; "Mapping of the Class II Region of the Human Major Histocompatibility Complex by Pulsed-Field Gel Electrophoresis"; Nature, vol. 323, pp. 453-455 (1986).
G. W. Slater and J. Noolandi; "New Biased-Reptation Model for Charged Polymers"; Physical Review Letters, vol. 55, No. 15, pp. 1579-1582 (1985).
M. Lalande, J. Noolandi, C. Turmel, R. Brousseau, J. Rousseau, G. W. Slater; "Scrambling of Bands in Gel Electrophoresis of DNA"; Nucleic Acids Research, vol. 16, pp. 5427-5437 (1988).
M. Lalande, J. Noolandi, C. Turmel, J. Rousseau, G. W. Slater; "Pulsed-Field Electrophoresis: Application of a Computer Model to the Separation of Large DNA Molecules"; Proceedings of the National Academy of Sciences USA, vol. 84, pp. 8011-8015 (1987).
G. W. Slater, J. Rousseau, J. Noolandi, C. Turmel, M. Lalande; "Quantitative Analysis of the Three Regimes of DNA Electrophoresis in Agarose Gels"; Biopolymers; 27, 509-524.
G. W. Slater and J. Noolandi; "The Biased Reptation Model of DNA Gel Electrophoresis"; New Trends in Physics and Physical Chemistry of Polymers, ed. by S. Lee (Plenum Press).
Noolandi Jaan
Slater Gary
Turmel Chantal
Palazzo E. O.
Starsiak Jr. John S.
Tung T.
Xerox Corporation
LandOfFree
Processes for the preparation and separation of macromolecules does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Processes for the preparation and separation of macromolecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Processes for the preparation and separation of macromolecules will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1204693