Gene amplification methods

Details Gene amplification methods Gene amplification methods

1996-05-01

1998-07-07

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435325, 435355, 435356, 435358, C12N 510, C12N 1569, C12N 1587

Patent

active

057767467

ABSTRACT:
The present invention provides improved methods for the amplification and expression of recombinant genes in cells. The methods of the present invention permit the isolation of cell lines which have co-amplified input recombinant sequences which encode an amplifiable marker, one or more expression vectors encoding a protein of interest and optionally a selectable marker. The present methods allow the efficient isolation of amplified cell lines which express the protein(s) of interest in a relatively short period of time. The present invention also provides compositions comprising amplified T lymphoid cell lines.

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