Process for the extraction and purification of proteins from cul

Details Process for the extraction and purification of proteins from cul Process for the extraction and purification of proteins from cul

1987-04-27

1989-08-15

Schain, Howard E.

Drug, bio-affecting and body treating compositions

Lymphokine

514 2, 530371, 530380, 530806, A61K 3929

Patent

active

048573175

ABSTRACT:
The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent; it comprises the precipitation of contaminants by polyethylene glycol and the treatment of this latter supernatant with either a bivalent metal cation or, after eventual ultrafiltration, with ammonium sulfate.

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Hitzeman et al., Nucl. Acids Res. 11:2745-2763 (1983).

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