Microorganism growth process and cell

Textiles: fiber preparation

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195100, 195102, 195139, C12B 100

Patent

active

040897486

ABSTRACT:
The invention is concerned with a micro-organism growth cell comprising a sterilized container having therewithin an agar-based nutrient gel. The gel has intimately intermixed therethroughout a specific amount of a dialkylpolysiloxane which causes a top surface of the gel to be smooth insuring a uniform growth rate for a micro-organism placed upon said top surface. The invention is also concerned with a process for growing a micro-organism upon the surface of an agar-based nutrient shell at a reproducibly constant growth rate. The process comprises intermixing with agar, essential nutrients and water, sufficient of said agar being used to insure formation of a gel, prior to the gelling thereof, a specific amount of a dialkylpolysiloxane. The resulting intermixture is gelled in a sterile micro-organism growth container to form a micro-organism growth cell as described above and the top surface of the gel is innoculated with a micro-organism. The cell is incubated under preselected incubation conditions whereby the micro-organism grows at a reproducably constant growth rate upon the smooth surface of the gel.

REFERENCES:
patent: 2819970 (1958-01-01), Steigmann
patent: 2894913 (1959-07-01), Sullivan et al.
Frobisher et al., "Fundamentals of Microbiology" Saunders Co., Ninth Edition, 1974, pp. 124-128.

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