Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1985-02-28
1989-04-25
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 26, 435 28, C12Q 126
Patent
active
048247790
ABSTRACT:
The present invention provides a method for the determination of NAD(P)H which comprises reacting a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z is hydrogen, hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino; and Z.sub.s in Z.sub.n may be the same or different, with NAD(P)H in the presence of (1) peroxidase or thiol oxide reductase and (2) diaphorase or an electron carrier, and determining the pigment thus formed.
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Chem. Abs. vol. 98, No. 17 (1983) 139879k.
Chem. Abs. vol. 90, No. 23 (1979) 182296q.
Pat. Abs. Japan, vol. 9, No. 222 (1945) 60-83598.
Aoyama Norihito
Miike Akira
Shimizu Yoshiaki
Tatano Toshio
Kyowa Medex Co., Ltd.
Warden Robert J.
Wieder Stephen C.
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