Enzyme immobilization with pullulan gel

Details Enzyme immobilization with pullulan gel Enzyme immobilization with pullulan gel

1978-02-16

1981-01-27

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 435181, C12N 1110

Patent

active

042476429

ABSTRACT:
Enzymes are immobilized by a process wherein the carrier used for immobilizing is a hydrophilic pullulan gel having a three-dimensionally reticulated structure obtained by the reaction between pullulan and a bifunctional compound capable of forming an ether linkage with the hydroxyl group contained in the glucose unit of pullulan, or an ionic pullulan gel obtained by the reaction between said hydrophilic pullulan gel and a compound having at one end an ionizable group and at the other end a functional group capable of forming an ether linkage with the hydroxyl group in the pullulan gel in the presence of an alkaline coumpound. Immobilized enzymes prepared according to this process have a high activity and good retention of the activity.

REFERENCES:
patent: 3645852 (1972-02-01), Axen et al.
patent: 3905954 (1975-09-01), Jones et al.
patent: 4090022 (1978-05-01), Tsao et al.
patent: 4152170 (1979-05-01), Nagase et al.

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