Method for the controlled synthesis of polynucleotide mixtures w

Details Method for the controlled synthesis of polynucleotide mixtures w Method for the controlled synthesis of polynucleotide mixtures w

1996-12-06

1998-08-04

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, 435 6, 935 78, 935 79, C07H 162, C07H 2100, C12H 1500, C12H 1510

Patent

active

057895775

ABSTRACT:
A method to obtain selected individual polynucleotides or mixtures thereof each of which encodes a peptide and at least one polynucleotide of the mixture encodes a peptide having a target property. The polynucleotides of the invention present in the mixture in detectable, retrievable, and clonable amounts are expressed in a host organism for screening for the target activity. The invention features the ability to synthesize controlled random polynucleotides to produce a predetermined mixture of polynucleotides and to avoid synthesis of a stop codon by adjusting the proportions into which the synthesis pool is subdivided and by adjusting the proportions of activated nucleotides added at each coupling step. A polynucleotide encoding a peptide having a target property can be selected and sequenced to deduce the amino acid sequence of the peptide.

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