Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1992-03-19
1992-11-17
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
435 79, 435174, 435964, 435972, 436512, 436547, 436548, 530402, 5303915, C12N 996, G01N 33535
Patent
active
051643111
ABSTRACT:
An antibody-enzyme conjugate is prepared having an enzyme to antibody ratio of approximately 3. The conjugate is produced by adding sulfhydryl groups to an antibody and maleimidyl groups to an enzyme to produce a modified antibody and enzyme, and reacting the modified antibody and enzyme to produce the conjugate. In producing the modified antibody and enzyme, about a 15 molar excess of reagents for introducing sulfhydryl and maleimidyl groups is used. When reacting the modified antibody and enzyme, a four molar excess of the modified enzyme is used, and reacting is stopped after a specified period of time by addition of selective reagents. The selective reagents may be cysteine and iodoacetamide.
REFERENCES:
patent: 4228237 (1980-10-01), Herey et al.
patent: 4684609 (1987-08-01), Hsu
patent: 4894229 (1990-01-01), Polson et al.
patent: 5002883 (1991-03-01), Bieniarz et al.
Imagawa et al., Journal of Applied Biochemistry, 4 (1982), pp. 41-57.
Cass Myron C.
Coulter Corporation
Naff David M.
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