Process and kit for detecting microbial metabolism

Details Process and kit for detecting microbial metabolism Process and kit for detecting microbial metabolism

1990-06-22

1992-11-17

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 34, 436172, 436800, 2504611, 2504612, C12Q 102

Patent

active

051643014

ABSTRACT:
A process and kit for detecting microbial metabolism is disclosed, the process including the steps of combining medium, a metabolic dye having an absorbance spectrum which in the presence of microorganisms changes and responds to metabolism of the microorganism and a second analytical dye having an excitation or emission spectrum which overlaps with one of the changed or unchanged absorbance spectra of the first dye. The medium contains a sample solution to be analyzed for containing metabolizing microorganisms. The user observes either no change in the fluorescence emissions of the second analytical dye indicating the absence of metabolizing microorganisms in the sample or a change indicating the presence of metabolizing microorganisms in the sample.

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