DNA-based diagnostic test for detecting myxobolus, the cause of

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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536 231, C12Q 168, C07H 2102

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059622270

ABSTRACT:
Compositions, methods and kits directed to detection of the myxozoan parasite Myxobolus spp., for example, M. cerebralis. The method comprises contacting an aquatic sample with a nucleic acid comprising a nucleic acid segment capable of selectively hybridizing to the 18S ribosomal nucleic acids of Myxobolus cerebralis to form a hybridization complex. Detection of the hybridization complexes indicates the presence of Myxobolus cerebralis in the sample.

REFERENCES:
Hendrick et al., "Three Myxosporeans Found in the Central and Brachial Tissues of Rainbow Trout in California," Journal of Aquatic Animal Health, vol. 3, pp. 55-62, 1991.
Andree et al., "Small Subunit Ribosomal RNA Sequences Unite Alternate Actinosporean and Myxosporean Stages of Myxobolus cerebralis the Causative Agent of Whriing Disease in Salmonid Fish," Journal of Eukaruotic Microbiology, vol. 44, Issue 3, pp. 208-215, May 1997.
Sommer and Tautz, "Minimal homology requirements for PCR primers," Nucleic Acids Research, vol. 17, No. 16, p. 6749, 1989.
Research Genetics "Designer PCR," (advertisement) Nucleic Acids Research vol. 22, No. 15, Aug. 1994.

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