Affinity chromatography of Vibrio cholerae enterotoxin-gangliosi

Details Affinity chromatography of Vibrio cholerae enterotoxin-gangliosi Affinity chromatography of Vibrio cholerae enterotoxin-gangliosi

1978-04-13

1980-09-30

Woodberry, Edward M.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

210656, 424 92, 536 1, C07G 700

Patent

active

042254876

ABSTRACT:
Columns of polysaccharide, e.g., agarose, derivatives comprising covalently bonded gangliosides quantitatively adsorb the .sup.125 I-labeled cholera toxin of chromatographed samples. The most preferred derivatives are those wherein the gangliosides are coupled to "macromolecules" [native albumin, denatured albumin, poly(L-lysine) and poly(L-lysyl-DL-alanine) graft copolymers] which themselves are covalently bonded to the, e.g., agarose. The soluble ganglioside polymers can prevent the binding of .sup.125 I-labeled toxin to liver membranes as well as block completely the lipolytic activity of cholera toxin on fat cells, and thus are useful in the management of the manifestations of clinical cholera.

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