Efficient process for isolating insoluble heterologous protein u

Details Efficient process for isolating insoluble heterologous protein u Efficient process for isolating insoluble heterologous protein u

1988-10-28

1992-01-21

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4353201, 435226, 435228, 43525233, 424 9466, 935 72, 935 73, 530412, C12N 121, C12N 1500, C12N 964, C12N 980, C12P 2100, A61K 3754, C07K 700

Patent

active

050827758

ABSTRACT:
The present invention relates to the isolation and restoration of biological activity to inactive proteins, that is solubilizing, renaturing and restoring activity to proteins which have been partially denatured or inactivated, e.g. during their synthesis in a host cell, such as E. coli, or during isolation. In particular this invention relates to both a means for extracting insoluble eukaryotic proteins from bacteria and to an efficient process for producing active chymosin from an insoluble chymosin precursor isolated from genetically engineered bacteria.

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