CDNA encoding a polypeptide including a hevein sequence

Details CDNA encoding a polypeptide including a hevein sequence CDNA encoding a polypeptide including a hevein sequence

1992-05-26

1995-03-21

Furman, Keith C.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530370, 530379, 435 697, 536 20, C07K 14415, C07K 1900

Patent

active

053996682

ABSTRACT:
A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

REFERENCES:
Riggs, P. D. 1990, "Expression and Purification of Maltose Binding Protein Fusions", in Current Protocols in Molecular Biology, (F. A. Ausubel et al., eds.) pp. 16.6.1-16.6.12, Green Publishing and Wiley Interscience, New York.
Van Parijs et al, 1991, Planta 183:258-264.
Lee et al. 1991, J. Biol. Chem. 266(24):15944-15948.
Broekaert, W, 1988, "Chitinases and Chitin-Binding Lectins in Plant: A Biochemical and Physiological Study of Their Role in the Natural Protection of Plants Against Fungi", Dessertationes de Agriculturo, Doctoraatsproefschrift Nr. 167 aan de Faauteit der Landbluvewetenschappen van de K. U. Leuven, pp. 42, 73-84 and the abstract.
Walujono, K., et al., in Proc. Internat. Rubber Conf. 518-531 (1975).
Wright, C. S., et al., Biochemistry 23, 280-287 (1984).
Raikhel & Wilkens, Proc. Natl. Acad. Sci. USA 84, 6745-6749 (1988).
Lerner, D. R., et al., Plant Physiol. 91, 124-129 (1989).
Wilkins, T. A., et al., Plant Cell 1, 541-549 (1989).
Chapot, M. P., et al., FEBS Lett. 195, 231-234 (1986).
Broglie, K. E., et al., Proc. Natl. Acad. Sci. USA 83, 6820-6824 (1986).
Shinshi, H. et al., Proc. Natl. Acad. Sci. USA 84, 89-93 (1987).
Parsons, T. J., et al., Proc. Natl. Acad. Sci. USA 86, 7895-7899 (1989).
Stanford, A., et al., Mol. Gen. Genet. 215, 200-208 (1989).
Weinstein, L. I., et al., Plant Physiol., 68, 358-363 (1981).
Coupe, M., et al. in Physiology of Rubber Tree Latex, eds. d'Auzac, J., et al. (CRC Press, Inc., FL) pp. 295-319 (1989).
Kush, A., et al., Proc. Natl. Acad. Sci. USA, 87 1787-1790 (1990).
Siflow, C. D., et al., Biochem. 18, 2725-2731 (1979).
Gubler, U., et al, Gene 25, 263-269 (1983).
Viera, J., et al., Methods Enzymol. 153 3-11 (1987).
Feinberg, A. P., et al. Anal. Biochem. 137, 266-267 (1985).
Raikhel, N. V., et al., Planta 176, 406-414 (1988).
Sanger, F., et al., Proc. Natl. Acad. Sci. USA 74, 5463-5467 (1977).
Mizusawa, S., et al., Nucl. Acids Res. 14, 1319-1324 (1986).
Dale, R. M. K., et al., Methods Enzymol. 155, 204-214 (1987).
Kirk, M. M., et al., Cell, 41, 419-428 (1985).
Molano et al, Anal. Biochem. 83, 648-656 (1977).
Boutry, M., et al., EMBO Journal 4, 2159-2165 (1985).
Guam et al, Gene 67, 21-30 (1988).
Hooft van Huijsduijnen, R. A. M., et al., Plant Mol. Biol. 9, 411-420 (1987).
Shinshi, H., et al., Plant Mol. Biol. 14, 357-368 (1990).
Broekaert, W. F., et al., Science 245, 1100-1102 (1989).
Schlumbaum, A., et al., Nature 324, 365-367 (1986).
Broekaert, W. F., et al. Physiol. Mol. Plant Pathol. 33, 319-331 (1988).
Graham, J. S., et al., Planta 169, 399-405 (1986).
Pena-Cortes, H., et al., Proc. Natl. Acad. Sci. USA 86, 9851-9855 (1989).
Boller, T., et al., Nature 286, 259-260 (1980).
Yang, S. F., et al. Annu. Rev. Plant Physiol. 35, 155-189 (1984).
Henstrand, J. M., et al, Plant Physiol. 91, 157-162 (1990).
Hedrick, S. A., et al., Plant Physiol. 86, 182-186 (1988).
Ecker, J. R., et al., Proc. Natl. Acad. Sci. USA 84, 5202-5206 (1987).

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