Method for enzymatic treatment of biofilm

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Cleaning using a micro-organism or enzyme

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D06M 1600

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active

061000809

ABSTRACT:
A method for cleaning and disinfecting a surface at least partly covered by a biofilm layer comprising the steps of contacting the biofilm with a cleaning composition comprising one or more hydrolases, e.g. a hydrolytic enzyme produced by a strain of the fungus Aspergillus aculeatus, in an amount effective for either fully or partly removing or releasing the biofilm layer from the surface; and contacting the biofilm with a bactericidal disinfecting composition comprising an oxidoreductase such as an oxidase, a peroxidase or a laccase, in an amount effective for killing the living bacterial cells present in the biofilm. In particular, a disinfecting composition comprising laccase at concentration between about 0.01 to about 1000 mg protein/ml composition and an oxidation enhancer such as methyl syringate.

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Dionysius et al., "Studies on the lactoperoxidase system: reaction kinetics and antibacterial activity using two methods for hydrogen peroxide generation", Journal of Applied Bacteriology, 1992, 72, pp. 146-153.
Brisou, J.F. 1995. Biofilm, methods for enzymatic release of microorganisms. CRC Press INc., Boca Raton, Fla. pp. 141-151, 173-176.
Russell et al. J. Agric. Food Chem. 1990, vol. 38, pp. 10-13.

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