Method for assaying enzyme activity using N-acetyl-.beta.-D-gluc

Details Method for assaying enzyme activity using N-acetyl-.beta.-D-gluc Method for assaying enzyme activity using N-acetyl-.beta.-D-gluc

1994-05-23

1995-03-21

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 536 173, 536 174, 536 175, 536 176, 536 172, 536 111, 536 41, 536 552, C12Q 134, C07H 510, C07H 1702, C07H 19048

Patent

active

053994884

ABSTRACT:
Novel N-acetyl-.beta.-D-glucosamine derivatives and a method for assaying N-acetyl-.beta.-D-glucosaminidase activity using the same as substrate are provided.
N-Acetyl-.beta.-D-glucosamine derivatives represented by general formula (I): ##STR1## wherein each of R.sub.1 and R.sub.2 independently represents hydrogen atom or methyl or ethyl group, are mixed with a sample containing N-acetyl-.beta.-D-glucosaminidase (NAG) and then absorbance of the reaction solution is determined to assay NAG activity extremely accurately.

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