Method of detecting substances by chemiluminescence

Details Method of detecting substances by chemiluminescence Method of detecting substances by chemiluminescence

1995-09-05

1998-04-07

Green, Lora M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 79, 435 14, 435 28, 435974, 436164, 436172, G01N 33535, C12Q 170

Patent

active

057363200

ABSTRACT:
A method of detecting substances, if desired in biological fluids, by using detection reactions based on chemiluminescence, wherein a luminophore, preferably having an emission wavelength longer than about 500 nm, is raised from its non-excited state into an excited state by electron transfer due to the detection reaction, and subsequently the radiation emitted by the luminophore when falling back into its non-excited state is measured. The chemiluminescence reaction is based on the oxidative decomposition of optionally substituted oxalates or oxamides, the radiation emitted by the luminophore when falling back into its non-excited state is of longer wavelength than the nonspecific background radiation of the reaction. The luminophore can be accumulated prior to the chemiluminesence reaction. Nonspecific background radiation of the reaction can be quenched.

REFERENCES:
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Grayeski et al., Anal. Biochem., vol. 136, pp. 277-284 (1984) "Determination of Fluorophor-Labeled Compounds Based on Peroxyoxalate Chemiluminescence".
Hemmila, Clin. Chem. vol. 31, No. 3, pp. 359-370 (1985) "Fluoroimmunoassays and Immunofluorometric Assays".
Irena Bronstein, Ph.D. et al, "Chemiluminescent Compounds for Diagnostic Tests," Research, 28:36-39 (1990).
Anthony K. Campbell et al., "A Homogeneous Immunoassay For Cyclic Nucleotides Based on Chemiluminescence Energy Transfer," Biochem. J., 216:185-194 (1983).
Katayama, M., et al., "Determination of Hydrogen Peroxide by Flow Injection Analysis with Aryl Oxalate-Sulforhodamine 101 Chemiluminescence", Analytical Letters, 24(6), pp. 1005-1015 (1991).
Takayasu, S., et al., "Chemiluminescent Enzyme Immunoassay Using .beta.-D-Galactosidase as the Label and the Bis(2,4,5-trichlorophenyl)oxalate-Fluorescent Dye System", Journal of Immunological Methods, 83, pp. 317-325 (1985).
Nakashima, K., et al., "High Performance Liquid Chromatography with Chemiluminescence Detection of Methamphetamine and its Related Compounds Using 4-(N,N-Dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole", Biomedical Chromatography, vol. 6, pp. 149-154 (1992).
Bador, R., et al., "Erythrosin as energy acceptor in a biphasic chemiluminescent system for glucose oxidase detection", Analytica Chimica Acta, 251, pp. 215-222 (1991).

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