Purification of active and inactive/latent forms of plasminogen

Details Purification of active and inactive/latent forms of plasminogen Purification of active and inactive/latent forms of plasminogen

1991-03-20

1993-11-09

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530395, 530416, C07K 302, C07K 322, C07K 1506, C07K 1514

Patent

active

052604195

ABSTRACT:
A method of separating active and inactive/latent forms of PAI-1 is disclosed comprising loading a sample containing a mixture of active and inactive/latent forms of PAI-1 to either an anion or cation exchange resin of 10-50 micron particle size and eluting active and inactive/latent forms of PAI-1 from the resin into separate fractions. Also disclosed is a method of extracting soluble E. coli-expressed recombinant PAI-1 from the lysed E. coli host cells in a buffered solution, wherein the ionic strength of the buffered solution is 8-25 millisiemens.

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Wun et al., FEBS Letters 210:11-16 (1987).
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Pannekoek et al., EMBO J. 5:2539-2544 (1986).
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