Purification of hapten-carrier generated antibodies

Details Purification of hapten-carrier generated antibodies Purification of hapten-carrier generated antibodies

1994-09-06

1996-12-31

Scheiner, Toni R.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, C07K 122, C07K 1600

Patent

active

055895751

ABSTRACT:
In the commonly used method to obtain antibodies to small molecules, a combination of a highly antigenic carrier, such as bovine serum albumin, and the small molecule is injected into a host animal. The recovered crude serum or plasma then contains, in addition to the desired small molecule antibodies, much larger amounts of carrier-induced antibodies. These unwanted antibodies are efficiently removed from the crude serum or plasma, by contacting the crude serum or plasma with the carrier material in an immobilized high surface area form. Rapid and efficient anticarrier antibody removal results, with minimal loss of both desired antibody, and desired antibody activity.

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Journal Of Immunology, vol. 116, No. 5, May 1976, Baltimore, U.S. pp. 1337-1341; D. S. Terman et al. "Specific Removal Of Bovine . . . ".

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