Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-08-15
1996-12-31
Knode, Marian C.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352401, C12N 500
Patent
active
055893767
ABSTRACT:
The invention includes methods for the isolation and clonal propagation of mammalian neural crest stem cells and isolated cellular compositions comprising the same. The methods employ a novel separation and culturing regimen and bioassays for establishing the generation of neural crest stem cell derivatives. These methods result in the production of non-transformed neural crest stem cells and their progeny. The invention demonstrates, at the clonal level, the self regeneration and asymmetrical division of mammalian neural crest stem cells for the first time in feeder cell-independent cultures. Lineage restriction is demonstrated within a developing clone and is shown to be sensitive to the local environment. Neural crest stem cells cultured on a mixed substrate of poly-D-lysine and fibronectin generate PNS neurons and glia, but on fibronectin alone the stem cells generate PNS glia but not neurons. The neurogenic potential of the stem cells, while not expressed, is maintained over time on fibronectin. The invention further includes transplantation assays which allow for the identification of mammalian neural crest stem cells from various tissues. It also includes methods for transplanting mammalian neural crest stem cells and/or neural or glial progenitors into mammals.
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Anderson David J.
Stemple Derek L.
California Institute of Technology
Duffy Patricia A.
Knode Marian C.
Silva Robin M.
Trecartin Richard F.
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