Method for enzymatic analysis and reagant therefor

Details Method for enzymatic analysis and reagant therefor Method for enzymatic analysis and reagant therefor

1994-11-03

1996-12-31

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435 25, 435 21, 435 23, 435 15, 435 791, 435190, 435194, 436 63, 436 74, 426 42, 426 52, C12Q 132, C12Q 100, G01N 3353, G01N 3348

Patent

active

055893490

ABSTRACT:
A method of enzymatic analysis utilizing a color-development signal amplification system associated with enzymatic cycling of NAD-NADH interconversion in the presence of dehydrogenase and its substrate, wherein the dehydrogenase is selected from the group consisting of alcohol dehydrogenase derived from Zymomonas and amino acid dehydrogenase derived from thermophilic microorganisms is disclosed. The use of alcohol dehydrogenase derived from Zymomonas provides an extremely higher detection sensitivity than that in the conventional method. The use of amino acid dehydrogenase derived from thermophilic microorganisms improves reliability of the method for a longer period of time than that in the conventional method.

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