High-throughput screening method for sequence or genetic alterat

Details High-throughput screening method for sequence or genetic alterat High-throughput screening method for sequence or genetic alterat

1994-07-28

1996-12-31

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 911, 435 912, C12Q 168, C12Q 170, C12P 1934

Patent

active

055893309

ABSTRACT:
The present invention pertains to high-throughput screening methods to identify genetic alterations in DNA samples. A multiplicity of DNA samples are immobilized on a solid support and hybridized simultaneously with a mixture of oligonucleotides representing variant sequences. Hybridizing oligonucleotides are then eluted from each DNA sample individually and their sequence is determined. The methods of the present invention allow the identification of disease-causing mutations and polymorphisms in patients' DNA, as well as the identification of disease-causing microorganisms.

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