Method and apparatus for effecting solid phase extraction

Details Method and apparatus for effecting solid phase extraction Method and apparatus for effecting solid phase extraction

1992-06-01

1993-11-09

Housel, James C.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

422 65, 422 67, 422101, 210635, 210641, G01N 3004, G01N 3014, G01N 3024

Patent

active

052600289

ABSTRACT:
A method and apparatus for practicing solid phase extraction of samples for analysis where the method comprises the steps of providing a cassette having a plurality of tubes, each containing an absorbent substrate and having an upper and a lower opening; applying a preconditioning liquid reagent to each tube of the cassette through the upper opening to render the substrate therein hydrophilic and drawing the liquid through the substrate until the liquid is at a predetermined height about the substrate; applying a liquid sample to be analyzed to each of the tubes of the cassette and drawing the sample through the substrate until the liquid is at a predetermined height in the tube with respect to the substrate; applying an eluate to each tube of the cassette to remove an eluent therefrom; and collecting the eluents from the cassette tubes. The apparatus automates this process and provides accurate and rapid separation of compounds in the sample. The process provides the further step of washing the substrate before addition of the eluate, if necessary, and optional drying of the substrate as well as addition of a second eluate to separate a second eluent if desired.

REFERENCES:
patent: 3578412 (1968-01-01), Martin
patent: 3883305 (1975-05-01), Hoskins et al.
Dimson, P. et al. "Automating Solid Phase Extraction for HPLC Sample Prep." American Laboratory, Oct. 1986 pp. 82-94.

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