Human endothelin converting enzyme obtained from blood or placen

Details Human endothelin converting enzyme obtained from blood or placen Human endothelin converting enzyme obtained from blood or placen

1994-08-10

1995-11-21

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 435226, C12N 948, C12N 950, C12N 964

Patent

active

054686334

ABSTRACT:
A human endothelin converting enzyme (ECE) has been isolated from blood and placenta. The enzyme has the activity of converting big endothelin-1, -2, or -3 to endothelin-1, -2, or -3 respectively. The enzyme has a mass of about 5.4.times.10.sup.5 by gel filtration, a pH optimum of 6.5 to 7.5, and a hydrated density of less than 1.210. The enzyme is inhibited by EDTA, phosphoramidon, thiorphan, chymostatin and phenylmethylsulfonylfluoride (PMSF). The enzyme is precipitated by 0.65% dextran sulfate or 0.2M manganese chloride. The enzyme can be inactivated by oxidation by copper ion.

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