Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism
Patent
1993-10-05
1995-05-02
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Treating animal or plant material or micro-organism
435 41, 435 681, 530356, C07K 114, C07K 14435, C07K 1478, A61K 3839
Patent
active
054118875
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to an improved way for the production of collagen and collagen produced through the process and the use of collagen in different areas.
PRIOR ART
Collagen is a fibre formed substance consisting of 18 amino-acids and it provides the main part of the intestines and stomach of animals but is also included in other parts of the animal body, for example in the interial part of the skin in the shape of hide, in lunges and udders etc. One has earlier primarily used the collagen in its native shape, mostly as sausage skin made from cleaned intestines. During resent years one has also produced collagen in its clean shape starting from the above said body parts of animals and used this clean collagen for different purposes such as artificially made sausage skins, additional agents for treatment articles, surgery threads etc. A known way of producing collagen is described in the Swedish Patent No. 8003876-3 in which a process is described that comprises cutting cleaned intestines etc. in stripes and pieces which are deep frozen and ground where upon they are subjected to the influence of an enzyme in a water solution whereby the aminoacids which do not provide a part of the collagen is hydrolysed so that insoluble remaining collagen can be removed and used after further treatment. Another process for producing collagen comprises the use of lime for separating the collagen fibrils. This process is time consuming and results also in that free collagen molecules are separated which impairs the properties of the end product.
TECHNICAL PROBLEM
It has since long been a desire to bring about a process which is better than the two above mentioned processes for the production of collagen. Thus, the first of said above mentioned processes includes that one has to freeze this disintegrated starting materials which is circumstantially and expensive and that one has to carry out the hydrolysis at a relatively low pH, approximately 8, using a relatively week enzyme during a period of 2-5 hours. This process is both costly and time consuming.
By the second of the above mentioned processes lime has to be added which results in a lower grade end product at the same time as an extended process will be necessary.
THE SOLUTION
By the present invention one has solved the problems connected with the above mentioned processes and brought about a process for the production of collagen from by-products of slaughtered and cut animals, such as intestines, stomach etc. which is characterized by the following process steps: regulated to approximately 5,5, further water is added so that the ground mixture contains approximately equal weight parts of starting materials and water, to at most 11, preferably 10,5 whereupon a proteolytic enzyme, for instance alkalas in an amount corresponding to 60 Anson-units per kilo solid substance is added so that hydrolysis of other proteins than collagen is carried out during maintaining the pH value by adding alkali until the hydrolyses is completed and alkali is no more consumed,
According to the invention it is suitable that pH in steps a) and d) is regulated by means of hydrochloric acid, citric acid or lactic acid.
The hydrolysing is carried out according to the invention during a period of time of 13/4 hours to 31/2 hours while adding sodium hydroxide as alkali.
According to the invention the separated collagen can be collected by sifting, cleaning with water at about 40.degree. C. and centrifugation or decanting. Only one wash with water is sufficient.
It is according to the invention further suitable that the collagen is homogenized after being collected at pH c:a 3 by alternatively adding acids and water while mechanically treating until the desired end concentration of collagen is obtained.
It is according to the invention suitable for obtaining a clear transparent film from the completed collagen that it is mixed with reduction agents, such as ascorbic acid and sodium sulphite in an amount of up to 2 weight per cent, and cross-binding agents for
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Derwent ABS 81-93071D/50 WO8103261, Sjoelander Nov. 26, 1981.
Derwent AB 93-312039/40 EP-564786, Coleman et al Oct. 1993.
Collagen Casing Einar Sjolander AB
Lilling Herbert J.
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