Method of quantitative assay for 1,5-anhydroglucitol

Details Method of quantitative assay for 1,5-anhydroglucitol Method of quantitative assay for 1,5-anhydroglucitol

1993-02-23

1995-11-21

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 14, 435 21, 435 28, 435 39, 435962, 514 23, C12Q 126, C12Q 128

Patent

active

054686210

ABSTRACT:
A quantitative assay for 1,5-anhydroglucitol in a specimen is described. This assay comprises treating a specimen with a phosphorylating enzyme in the presence of 5 to 500 mM adenosine-5'-triphosphate in a pH range of 7.2 to 8.5, to selectively eliminate sugars other than 1,5-anhydroglucitol, and reacting the product of this reaction, without removing the residual ATP, with pyranose oxidase in the above pH range. This assay is quite rapid and suitable for performance using an automated device.

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Fukumura, Yukihito et al., "Interference of Maltose for the Determination of 1,5-Anhydroglucitol with Lana AG.sup..epsilon. Kit", Rhinsho Kagku (Nippon Rinsho Kagaki), 21 (1992), pp. 43-48.
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