Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-08-18
1995-11-21
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 724, 435 725, 436518, 436519, 436520, G01N 33543, G01N 33554, G01N 33555
Patent
active
054686180
ABSTRACT:
An article for performing solid-phase immunological assays and methods for performing assays utilizing such article. A solid-phase support suitable for immunological assays is stained with an organic dye that has a net-positive charge and a hydrophobic aromatic ring structure. An immunologically reactive component having a net-negative charge, such as a whole cell, is securely immobolized by the dye to the support through noncovalent interactions. A sample of biological fluid may be added to the article to which the immunologically reactive component has been immobilized to effect binding of antigens or antibodies in the sample which are specific to the immobilized component. The presence of the antigen-antibody complex may then be determined by any known means.
REFERENCES:
patent: 3619371 (1971-11-01), Crook et al.
patent: 3646346 (1972-02-01), Catt
patent: 4108974 (1978-08-01), Wegfahrt et al.
patent: 4331759 (1982-05-01), Giannini et al.
patent: 4360358 (1982-11-01), Sharma
patent: 4373932 (1983-02-01), Gribnau et al.
patent: 4390518 (1983-06-01), Fischer et al.
patent: 4714606 (1987-12-01), Kass
patent: 4783401 (1988-11-01), Horan et al.
patent: 4786595 (1988-11-01), Arai et al.
patent: 4792521 (1988-12-01), Shochat
patent: 4853186 (1989-08-01), Mura et al.
patent: 4853210 (1989-08-01), Kass
patent: 4855240 (1989-08-01), Rosenstein et al.
patent: 4939085 (1990-07-01), Arai
patent: 5047321 (1991-09-01), Loken et al.
"Depletion by monolayer binding of specific precursors of antibody-forming cell directed against cellular antigens", A. D. Nash and W. Boyle, Immunology 1986, 57, pp. 411-417.
"Immunofluorescence with Monoclonal Antibodies on Poly-L-Lysine Coated Slides: An Alternative to Conventional Methods", M. M. Mehta, R. Munker, B. Kranz, E. Thiel and S. Thierfelder, Blut (1983) 47:237-242.
"An Improved Assay for Haemolytic Plaque-Forming Cells", J. C. Kennedy and M. A. Axelrad, Immunology, 1971, 20, 253-257.
"A new method for the irreversible attachment of cells or proteins to polystyrene tissue culture plates for use in the study of bacterial adhesion", D. W. McEachran and R. T. Irvin, Journal of Microbiological Methods 5 (1986) 99-111.
"Use of Glutaraldehyde ad a Coupling Agent for Proteins and Peptides", Morris Reichlin, Methods of Enzymology 1980: 70; 159-165.
"Erythrocyte Adhesion to Polymer Surfaces", D. R. Absolom W. Zingg, C. Thomson, Z. Policova, C. J. Van Oss, and A. W. Neumann, Journal of Colloid and Interface Science, vol. 104, No. 1, Mar. 1985, pp. 51-59.
"Preparing Monolayers of Non-Adherent Mammalian Cells", M. Jules Mattes, Mitsune Tanimoto, Marilyn S. Pollack, and David H. Maurer, Journal of Immunological Methods, 61 (1983), 145-150.
"Method for Binding Cells to Plastic: Application to Solid Phase Immunoassays for Cell-Surface Antigens", C. H. Heusser, J. W. Stocker, R. H. Gisler, Methods of Enzymology, vol. 78, pp. 406-418.
"Preparation of Immobilized Animal Cells", Kjell Nilsson and Klaus Mosbach, FEBS Letters,vol. 118, No. 1, Aug. 1980, pp. 145-150.
"Adhesion of Cells to Polystyrene Surfaces", A. S. G. Curtis, J. V. Forrester, C. McInnes, and F. Lawrie, The Journal of Cell Biology, vol. 97, Nov. 1983, pp. 1500-1506.
"Entrapment of Enzymes and Microorganisms in Synthetic Cross-linked Polymers and their Application in Column Techniques", Klaus Mosback and Rolf Mosbach, Acta Chemical Scandinavia 20 (1966), No. 10, pp. 2807-2810.
"Chemical Activities of E. Coli Adsorbed on a Resin", Tsutomo Hattori and Choseki Furusaka, The Journal of Biochemistry, vol. 48, No. 6, 1960, p. 831.
Biological Stains, H. J. Conn. ed. R. D. Lille, M.D., 9th Ed.
Eatz Ralph A.
Plapp Fred V.
Sinor Lyle T.
Stone Darryl L.
Ceperley Mary E.
Immucor, Inc.
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