Method for retrieval of unknown flanking DNA sequence

Details Method for retrieval of unknown flanking DNA sequence Method for retrieval of unknown flanking DNA sequence

1991-11-01

1995-05-02

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 435810, 536 221, 536 242, 536 243, 935 77, 935 78, 935 88, C12P 1934

Patent

active

054118751

ABSTRACT:
A method that permits the highly specific PCR amplification of unknown DNA that flanks a known sequence region. In this method, known DNA is placed on the uncharacterized side of that specific sequence that contains the unknown DNA by a DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplification of the segment of interest.

REFERENCES:
Jones et al. (1992) Nucleic Acids Research, vol. 20, No. 3, pp. 595-600.
Cariello et al. (1991) Gene, vol. 99, pp. 105-108.

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