Process for the purification of carbohydrate containing enzymes

Details Process for the purification of carbohydrate containing enzymes Process for the purification of carbohydrate containing enzymes

1978-07-31

1980-03-18

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435192, 435189, 435262, 435814, C07G 700

Patent

active

041940679

ABSTRACT:
This invention relates to a method for purifying a carbohydrate containing enzyme which is a desired enzyme preferred to be separated from a mixture. The method comprises the steps of mixing a solution containing the carbohydrate containing enzyme with a carbohydrate modifying reagent. The carbohydrate modifying reagent reacts with the carbohydrate attached to the enzyme, thereby modifying its chemical structure. The modified enzyme is then separated from the other undesirable enzymes or proteins in the mixture by a suitable chemical separation method, for example, gel filtration chromatography. The method was used in the separation of glucose oxidase from catalase, a separation which by previous methods was very inefficient.

REFERENCES:
Nakamura et al., in FEBS Letters, vol. 41, No. 2, pp. 327-330, May 1974.

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