Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence
Patent
1998-04-21
1999-08-10
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
25 or more amino acid residues in defined sequence
530326, 530333, 530344, 514 12, 514 13, 514 21, 424551, A61K 3800
Patent
active
059360631
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a novel antimicrobial peptide isolated from Bufo bufo gargarizans, more specifically, to a novel antimicrobial peptide isolated from Bufo bufo gargarizans which possesses strong antibacterial and antifungal activities.
2. Description of the Prior Art
Most higher organisms accumulate or secrete antimicrobial peptides as defensive mechanism to protect themselves against pathogenic microorganisms such as virus and bacteria, which also appears in invertebrates including insects. In this connection, studies on the antimicrobial peptides such as Cecropin, an antibiotic peptide isolated from larva of silkworm, have been actively carried out in the art.
Until now, 2,000 or more species of antimicrobial peptides have been discovered, which have different amino acid composition depending on their origin, while they show similar mode of action. These antimicrobial peptides have attracted a considerable attention as new proposed medicines, grounded on the following advantages: first, the antimicrobial peptides have strong activities against broad spectrum of microorganisms, when compared with the conventional antibiotics; secondly, they are potential antibiotics useful for the treatment of human body, since they show antimicrobial activities only against infective pathogens without harmful effect on host cells; thirdly, they can be prepared massively from prokaryotes by the aid of recombinant DNA technology, since all of them do not undergo translational modification such as glycosylation, etc.
Recently, it has been reported that several kinds of antimicrobial peptides exist in skin and stomach of amphibia, some of examplaries are Cecropin, Defensin, Magainin, and Tachyplesin, etc. which consist of 17 to 24 amino acids. They show antimicrobial activities against fungi as well as gram-negative and positive bacteria, and some of them are effective against cancer cell and virus.
Magainin is a famous antibiotic peptide consisting of 23 amino acids, which acts on human pulmonary carcinoma cell as well as pathogenic microoganisms (see: Michael A., Proc. Natl. Acad. Sci. USA, 84:5449-5453 (1987)), and it has been proposed as the most applicable antibiotic peptide now, based on the studies on its antimicrobial activity, mode of action, and structure. In accordance with the present invention, the activities of the antimicrobial peptide of the invention were compared with those of Magainin.
SUMMARY OF THE INVENTION
The present inventors have made an effort to seek novel antibiotic substances from amphibia which have been known to possess a lot of antimicrobial peptides, and isolated a novel antimicrobial peptide from a Korean toad, Bufo bufo gargarizans, which shows stronger antibacterial and antifungal activities than any other conventional antimicrobial peptides.
A primary object of the invention is, therefore, to provide a novel antimicrobial peptide isolated from Bufo bufo gargarizans.
BRIEF DESCRIPTION OF THE DRAWINGS
The above and the other objects and features of the present invention will become apparent from the following descriptions given in conjunction with the accompanying drawing, in which:
FIG. 1 is mass spectrum which determines molecular weight of the antimicrobial peptide of the invention.
DETAILED DESCRIPTION OF THE INVENTION
In Korea, Bufo bufo gargarizans, a Korean toad has been used as a folk medicine for the treatment of external wound in a dried powder-like formulation. In this connection, the present inventors have screened novel antibiotic substances from the amphibia including Bufo bufo gargarizans which has been known to possess a lot of antimicrobial peptides. The present inventors first homogenized stomachs of Bufo bufo gargarizans, extracted and concentrated peptides only, and carried out heparin-Sepharose chromatography and C.sub.18 reverse-phase HPLC (high performace liquid chromatography).
Determination of molecular weight and amino acid sequence of the purified antimicrobial peptide were followed t
REFERENCES:
patent: 3342812 (1967-09-01), Komatsu et al.
Park, et al., "A Novel Antimicrobial Peptide from Bufo bufo gargarizans", Biochem. Biophys. Res. Commun., 1996, 218, 408-413.
M. Zasloff, Proc. Nat'l. Acad. Sci., USA, 84:5449-5453, Aug. 1987.
Hong Seung-Suh
Kim In-Cheol
Kim Sun-Chang
Lee Dong-Kyun
Lee Hyun-Soo
Jameison Fabian A.
Samyang Genex Co., Ltd.
Tsang Cecilia J.
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