Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-03-24
1994-09-13
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 435221, 43525231, 4353201, 536 232, 935 14, 935 29, 935 74, 935 10, C12N 1500, C12N 1557, C12N 1575, C12N 1511
Patent
active
053468236
ABSTRACT:
There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.
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Bott Richard R.
Estell David A.
Wells James A.
Genencor, Inc.
Horn Margaret A.
Moore William W.
Wax Robert A.
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