Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-11-30
1999-08-10
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352523, 43525231, 43525233, 43525234, 43525235, 4352542, 43525421, 43525422, 43525423, 4353201, 435348, 435355, 435357, 435364, 435365, 536 235, 536 234, 536 2431, C12N 1512, C12N 115, C12N 121, C12N 510
Patent
active
059358106
ABSTRACT:
The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of weight, and to the diagnostic and therapeutic uses to which such modulators may be put. In its broadest aspect, the present invention relates to the elucidation and discovery of nucleotide sequences, and proteins putatively expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian body weight. The nucleotide sequences in object represent the genes corresponding to the murine and human ob gene, that have been postulated to play a critical role in the regulation of body weight and adiposity. Preliminary data, presented herein, suggests that the polypeptide product of the gene in question functions as a hormone. The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i.e., synthetic or natural oligonucleotides. In further aspects, the present invention provides a cloning vector, which comprises the nucleic acids of the invention; and a bacterial, insect, or a mammalian expression vector, which comprises the nucleic acid molecules of the invention, operatively associated with an expression control sequence. Accordingly, the invention further relates to a bacterial or a mammalian cell transfected or transformed with an appropriate expression vector, and correspondingly, to the use of the above mentioned constructs in the preparation of the modulators of the invention. Also provided are antibodies to the ob polypeptide. Moreover, a method for modulating body weight of a mammal is provided. In specific examples, genes encoding two isoforms of both the murine and human ob polypeptides are provided.
REFERENCES:
patent: 5521283 (1996-05-01), DiMarchi et al.
patent: 5525705 (1996-06-01), DiMarchi et al.
patent: 5532336 (1996-07-01), DiMarchi et al.
patent: 5552522 (1996-09-01), DiMarchi et al.
patent: 5552523 (1996-09-01), Basinski et al.
patent: 5552524 (1996-09-01), Basinski et al.
patent: 5554727 (1996-09-01), Basinski et al.
patent: 5559208 (1996-09-01), Basinski et al.
patent: 5563243 (1996-10-01), DiMarchi et al.
patent: 5563244 (1996-10-01), DiMarchi et al.
patent: 5563245 (1996-10-01), DiMarchi et al.
patent: 5567678 (1996-10-01), DiMarchi et al.
patent: 5567803 (1996-10-01), Basinski et al.
patent: 5569743 (1996-10-01), DiMarchi et al.
patent: 5569744 (1996-10-01), Basinski et al.
patent: 5574133 (1996-11-01), DiMarchi et al.
patent: 5580954 (1996-12-01), DiMarchi et al.
patent: 5594101 (1997-01-01), Becker et al.
patent: 5594104 (1997-01-01), Basinski et al.
patent: 5605886 (1997-02-01), Basinski et al.
patent: 5614379 (1997-03-01), MacKellar et al.
patent: 5643748 (1997-07-01), Snodgrass et al.
patent: 5691309 (1997-11-01), Basinski et al.
patent: 5698389 (1997-12-01), de la Brousse
Fletcher et al., Experimental Hematology 24(9):1055, abstract 172 (1996).
Fletcher et al., Blood 86(10 Suppl. 1):241A, abstract 951 (1995).
Bahary et al., 1993, Mammalian Genome 4:511-15.
Bahary et al., 1993, Genomics 16:113-22.
Friedman and Leibel, 1992, Cell 69:217-20.
Bahary et al., 1992, Genomics 13:761-69.
Friedman et al., 1991, Ann. NY Acad. Sci. 630:100-115.
Friedman et al., 1991, Mammalian Genome 1:130-44.
Friedman et al., 1991, Genomics 11:1054-62.
Moll et al., 1991, Am. J. Hum. Genet. 49:1243-55.
Truett et al., 1991, Proc. Natl. Acad Sci. USA 88:7806-09.
Walther et al., 1991, Genomics 11:424-34.
Bahary, 1990, Proc. Natl. Acad. Sci. USA 87:8642-46.
Harris, 1990, FASEB J. 4:3310-18.
Leibel et al., 1990, "Genetic variation and nutrition in obesity: Approaches to the molecular genetics of obesity", World Review of Nutrition and Dietetics, vol. 63 (Simopoulos, A.P. and Childs, B., eds.), pp. 90-101.
Bray, 1989, Am. J. Clin. Nutr. 50:891-902.
Harris et al., 1987, Diabetes 36:523-34.
Bogardus et al., 1986, N. Engl. J. Med. 315:96-100.
Keesey and Powley, 1986, Ann. Rev. Psychol. 37:109-33.
Coleman, 1978, Diabetologia 14:141-48.
Coleman, 1973, Diabetologia 9:294-98.
Hervey, 1959, J. Physiol. 145:336-52.
Dickie and Lane, 1957, Mouse News Lett. 17:52.
Ingalls et al., 1950, J. Hered. 41:317-18.
Burley Stephen K.
Friedman Jeffrey M.
Gajiwala Ketan
Halaas Jeffrey L.
Maffei Margherita
Railey II Johnny F.
The Rockefeller University
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