Method and apparatus for speciating and identifying MAI (Mycobac

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 4, 435 32, 435 29, 4352871, 4352991, 4352879, 4352883, 4352881, 4352862, 936 63, C12Q 104

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active

059358068

ABSTRACT:
A method of speciating and identifying MAI in a specimen comprises placing a paraffin coated slide in a receptacle containing a sterile aqueous solution inoculated with the specimen, analyzing the slide after exposure to the specimen to determine the presence or absence of atypical Mycobacteria, and after the analysis step, if atypical Mycobacteria are determined to be present, performing at least one speciation assay to ascertain if the atypical Mycobacteria are MAI. A related apparatus is also disclosed for speciating and identifying MAI in a specimen comprising a paraffin-wax coated slide, a tube having a sterile aqueous solution contained therein, the tube adapted to hold the slide, and at least one speciation assay means for performing an assay to determine the presence or absence of MAI in the specimen after the specimen is introduced into the tube holding the solution and the slide. An apparatus and method for determining the sensitivity of MAI to different antibiotics and dosages thereof is also provided.

REFERENCES:
patent: 4683201 (1987-07-01), Hamill
patent: 5316918 (1994-05-01), Ollar
patent: 5569592 (1996-10-01), Ollar
patent: 5698414 (1997-12-01), Ollar
Ollar R. Zbl. Bakt. I. Abt. Org. A. 234. pp. 81-90 (1976).
Fuhs, G.W., "Der Mikrobielle Abbau Von Kohlenwasserstoffen", Arch. Mikrobiol., 39:374-422 (1961).
Mishra, S.K. et al., "Observations On Paraffin Baiting As A Laboratory Diagnostic Procedure in Nocardiosis", Mycopathologica and Mycologia Applicatia 51 (2-3):147-157 (1973).
Ollar, R.-A., "A Paraffin Baiting Technique that Enables a Direct Microscopic View of "in situ" Morphology of Nocardia asteroides with the Acid-Fast or Fluorescence Staining Procedures", Zbl. Bakt. Hyg., Abt. Orig. A, 234:81-90 (1976).
Kirihara, J.M. et al., "Improved Detection Times for Mycobacterium avium Complex and Mycobacterium tuberculosis with BACTEC Radiometric System", J. Clin. Microbiol. 22:841-845 (1985).
Gonzalez, R. et al., "Evaluation of Gen-Probe DNA Hybridization Systems for the Identification of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare", Diagn. Microbiol. Infect. Dis. 8:69-77 (1987).
Wallace, J.M. et al., "Mycobacterium avium Complex Infection in Patients with Acquired Immunodeficiency Syndrome -- A Clinicopathologic Study", Chest 93(5): 926-932 (1988).

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