Method of DNA base sequence determination

Details Method of DNA base sequence determination Method of DNA base sequence determination

1997-11-12

1999-08-10

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435973, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 436527, 436518, 436809, C12Q 168, C12P 1934, C07H 2102, G01N 33533

Patent

active

059357940

ABSTRACT:
The method of base sequence determination according to the present invention ensures an effective determination of a long DNA base sequence, by providing simultaneous determination of base sequences of two or more positions of the long DNA or base sequences of two or more DNAs, using the DNA probe chip which classifies and retains the DNA oligomers having various sequences, and using fluorophorelabeled primers which have the same sequencies as the oligomers in the chip and are labeled by various fluorophores, then followed by the extension of the determined base length by re-selection of the primers complementary to the sequence thus determined.

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Matthews et al, "Analytical strategies for the use of DNA probes", Anal. Biochem. 169:1-25, 1988.
Matera et al, "A transpositionally and transcriptionally competent Alu subfamily", Mol. Cell. Biol. 10(10):5424-5432, Oct. 1990.

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