Isoelectric focusing method

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

204180P, 204299R, 204301, B01D 1302, G01N 2726

Patent

active

042049296

ABSTRACT:
The disclosure is directed to a method and apparatus for isoelectric focusing of fluids. In accordance with the disclosed method, the flow of fluids to be processed is established in a first direction. This flow of fluids is streamlined by providing a plurality of permeable microporous membranes which define generally parallel channels oriented in the first direction. An electrical potential is applied across the streamlined channels of flowing fluid, and isoelectric focusing is achieved on the fluids during the flow thereof since the membranes allow interchange of fluid constituents therebetween while providing the desired streamlining. An approximation of "plug" type flow is achieved within the streamlined channels; i.e., flow having an approximately uniform cross-sectional characteristic. In the preferred embodiment of the method of the invention, a recirculation path is established for each of the streamlined channels, such that the fluid flowing out of each channel is recirculated back to the beginning of the channel. Preferably, this is achieved by pumping the fluids in each of the recirculation paths, and also providing cooling for the fluids during the recirculation thereof. In this manner, a number of "passes" are effected to obtain the desired degree of isoelectric focusing. The cooling, which is performed during the recirculation, serves to minimize problems with dissipation of Joule heat during the isoelectric focusing of the fluids in the streamlined channels. Also, in the preferred embodiment of the invention, the first direction is downward such that the streamlined fluids flow under the influence of gravity to permit gravity equilibrium of fluid levels in the channels across the streamlining membranes.

REFERENCES:
patent: 2878178 (1959-08-01), Bier
patent: 3079318 (1963-02-01), Bier
patent: 3677923 (1972-07-01), Bier
patent: 3751356 (1973-08-01), Takeya et al.
patent: 3972791 (1976-08-01), Stern
patent: 3989613 (1976-11-01), Gritzner
patent: 4040940 (1977-08-01), Bier
Bier "Membrane Process for Industry," Proceedings of Symposium of Southern Research Institute, 1966, "Forced Flow Electrophoresis . . . ".
Snyder et al., "New Electrochemical Process for Treating Spent Emulsions," General Motors Research, GMR-2097, 1976.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Isoelectric focusing method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isoelectric focusing method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isoelectric focusing method will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1118615

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.