DNA sequencing

Details DNA sequencing DNA sequencing

1992-09-24

1994-09-13

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041801, 2041828, C25B 900

Patent

active

053466039

ABSTRACT:
To sequence long strands of DNA, cloned strands having lengths longer than 100 bases are, in one embodiment, marked on one end with biotih. These strands are divided into 4 aliquots and each aliquot: (1) is uniquely chemically treated to randomly terminate the strands at the non-biotinylated end at a selected type of base; and (2) is moved continuously by electrophoresis through a different one of four identical channels. In the one embodiment, the strands are randomly terminated at a selected base type and they are moved into avidin, which due to high affinity, combines with the biotin marked ends of shorter strands before the longer strands are fully resolved in the gel. The avidin is marked with fluorescein, the strands are scanned and the signals are decoded. In another embodiment, the strands are synthesized, with termination at a selected base type and marked either by the above method or by ethidium bromide.

REFERENCES:
patent: 3640813 (1972-02-01), Nerenberg
patent: 3980546 (1976-09-01), Caccavo
patent: 4111785 (1978-09-01), Roskam
patent: 4284491 (1981-08-01), Vesterberg
patent: 4431506 (1984-02-01), Gorman, Jr. et al.
patent: 4689295 (1987-08-01), Taber et al.

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