Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-05-09
1999-02-02
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 911, 435 912, 435 913, 536 231, 536 243, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
058663371
ABSTRACT:
The present invention provides a method for detecting a mutation in a nucleic acid molecule which comprises contacting the nucleic acid molecule with a probe. The probe comprises two covalently linked nucleic acid segments under conditions such that the unlinked end of each segment of the probe is capable of hybridizing with the nucleic acid molecule. This mixture is then contacted with a ligase under conditions such that the two hybridized probe segments will ligate and bind the nucleic acid molecule if the nucleic acid molecule contains the mutation. One would then determine the presence of bound nucleic acid molecule(s) and thereby detect the mutation in the nucleic acid molecule.
REFERENCES:
patent: 4959312 (1990-09-01), Sirotkin
Matthews, (1988), "Analytical strategies for the use of DNA probes", Anal. Biochem. 169: 1-25.
Stein et al, (1988), "Oligodeoxynucleotides as inhibitors of gene expression: a review", Cancer Research 48: 2659-2668.
Fredman Jeffrey
Jones W. Gary
The Trustees of Columbia University in the City of New York
White John P.
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