Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-03-01
1999-02-02
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 2433, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104
Patent
active
058663339
ABSTRACT:
The expression of APOBEC-1 in the liver of transgenic mice can cause liver dysplasia and liver tumors, and the expression of human APOBEC-1 in the intestine, stomach, or brain can cause obesity. Promiscuous editing of other mRNAs is correlated to these phenotypic changes. The instant invention discloses novel techniques for detecting aberrantly edited mRNAs, and hence, genes responsible for non-wild type phenotypes.
REFERENCES:
patent: 5389514 (1995-02-01), Taylor
patent: 5434058 (1995-07-01), Davidson
patent: 5550034 (1996-08-01), Teng et al.
patent: 5643778 (1997-07-01), Nishikura
Davies et al. (1989) J. Biol. Chem. 264:13395-13398.
Navaratnam et al. (1991) Nucl. Acids Res. 19:1741-1744.
Davidson et al. (1988) J. Biol. Chem. 263:13482-13485.
Shaugnhessy, J.D. et al., "The mammalian homolog of the p82 subunit of wheat eIF-(iso)4F: cDNA cloning, expression analysis, and chromosomal localization," Genebank Accession No. U63323 (1996).
Backus, et. al. "Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited", Biochem. Biophys. Acta. 1217: 65-73 (1994).
Backus, et. al. "Specific 3' sequences flanking a minimal apolipoprotein B (apoB) MRNA editing `cassette` are critical for efficient editing in vitro", Biochem. Biophys. Acta. 1219: 1-14 (1994).
Campfield, et. al. "Recombinant Mouse OB Protein: Evidence for a Peripheral Signal Linking Adiposity and Central Neural Networks", Science 269: 546-549 (1995).
Chua, et. al. "Phenotypes of Muose diabetes and Rat fatty Due to Mutations in the OB (Letpin) Receptor", Science 271: 994-996 (1996).
Davidson, et. al. "Proposed nomenclature for the catalytic subunit of the mammalian apolipoprotein B mRNA editing enzyme: APOBEC-1", RNA 1:3 (1995).
Driscoll, et. al. "Induction of RNA Editing at Heterologous Sites by Sequences in Apolipoprotein B mRNA", Mol. Cell. Biol. 13: 7288-7294 (1993).
Friedman, et. al. "Molecular Mapping of the Mouse ob Mutation", Genomics 11: 1054-1062 (1991).
Hadjiagapiou, et. al. "Molecular cloning of a human small intestinal apolipoprotein B mRNA editing protein", Nucleic Acids Res. 22: 1874-1879 (1994).
Halaas, et. al. "Weight-Reducing Effects of the Plasma Protein Encoded by the obese Gene", Science 269: 543-546 (1995).
Lau, et. al. "Dimeric structure of a human apolipoprotein B mRNA editing protein and cloning and chromosomal localization of its gene", Proc. Natl. Acad. Sci. U.S.A. 91: 8522-8526 (1994).
Liang & Pardee "Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction", Science 257: 967-971 (1992).
Nakamuta, et. al. "Alternative mRNA Splicing and Differential Promoter Utilization Determine Tissue-specific Expression of the Apolipoprotein B mRNA-editing Protein (Apobec1) Gene in Mice", J. Biol. Chem. 270: 13042-13056 (1995).
Pelleymounter, et. al. "Effects of the obese Gene Product on Body Weight Regulation in ob/ob Mice", Science 269: 540-543 (1995).
Reeves, et. al. "General Method for PCR Amplification and Direct Sequencing of mRNA Differential Display Products", Biotechniques 18: 18-20 (1995).
Shah, et. al. "Sequence Requirements for the Editing of Apolipoprotein B mRNA", J. Biol. Chem. 266: 16301-16304 (1991).
Tartaglia, et. al. "Identification and Expression Cloning of a Leptin Receptor, OB-R", Cell 83: 1263 (1995).
Teng, et. al. "Molecular Cloning of an Apolipoprotein B Messenger RNA Editing Protein", Science 260: 1816-1819 (1993).
Wang & Feuerstein "Direct Sequencing of DNA Isolated from mRNA Differential Display", Biotechniques 18: 448-452 (1995).
Yamanaka, et. al. "Cloning and Mutagenesis of the Rabbit ApoB mRNA Editing Protein", J. Biol. Chem. 269: 21725-21734 (1994).
Yamanaka, et. al. "Apolipoprotein B mRNA-editing protein induces hepatocellular carcinoma and dysplasia in transgenic animals", Proc. Natl. Acad. Sci. U.S.A. 92: 8483-8487 (1995).
Zhang, et. al. "Positional cloning of the mouse obese gene and its human homologue", Nature 372: 425-432 (1994).
Innerarity Thomas L.
Qian Xiaobing
Yamanaka Shinya
Fitts Renee A.
Regents of the University of California
Zitomer Stephanie W.
LandOfFree
Screening methods to detect mRNA targets of editing enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Screening methods to detect mRNA targets of editing enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Screening methods to detect mRNA targets of editing enzymes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1116137