Protein having .alpha.-glucosidase activity, DNA having genetic

Details Protein having .alpha.-glucosidase activity, DNA having genetic Protein having .alpha.-glucosidase activity, DNA having genetic

1996-03-06

1998-08-18

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435201, 4352523, 43525411, 435243, 435325, 435410, 536 232, C12N 928, C12N 1556, C12N 1563, C12N 510, C12N 100

Patent

active

057957665

ABSTRACT:
A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No. 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.

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