Methods for the assay of troponin I and T and selection of antib

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 72, 435 75, 435 771, 435 772, 435 78, 435 79, 435 792, 435 793, 435 794, 435 795, 435960, 435962, 435971, 435973, 436518, 436523, 436528, 436535, 436538, 436811, 436819, G01N 33553

Patent

active

057957258

ABSTRACT:
Assays and antibodies are disclosed for the detection and quantitation of cardiac specific troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I and T and the binary and ternary complexes may be related to the metabolic state of the heart. More specifically, immunoassays for determining the presence or amount of free, binary and ternary complexes of troponin I and T are claimed.

REFERENCES:
patent: 4933275 (1990-06-01), Wands et al.
patent: 5051356 (1991-09-01), Warren, III et al.
patent: 5143852 (1992-09-01), Valkirs et al.
patent: 5210020 (1993-05-01), Kondo et al.
patent: 5246001 (1993-09-01), Ohman et al.
patent: 5290678 (1994-03-01), Jackowski
patent: 5447846 (1995-09-01), Shinoki et al.
patent: 5604105 (1997-02-01), Jackowski
Anderson et al., "Troponin T Isoform Expression in Humans," Circulation Research 69(4):1226-1233 (1991).
Ball et al., "Isoform Specific Interactions of Troponin I and Troponin C Determine pH Sensitivity of Myofibrillar Ca.sup.2+ Activation," Biochemistry 33:8464-8471 (1994).
Bhayana et al., "Discordance Between Results for Serum Troponin T and Troponin I in Renal Disease," Clin. Chem. 41(2):312-317 (1995).
Bhavsar et al., "Developmental expression of troponin I isoforms in fetal human heart," FEBS Letters 292:(1,2):5-8 (1991).
Blechner et al., "4Ca.sup.2+ .multidot.Troponin C Forms Dimers in Solution at Neutral pH that Dissociate upon Binding Various Peptides: Small-Angle X-ray Scattering Studies of Peptide-Induced Structural Changes," Biochemistry 31:11326-11334 (1992).
Borrebaeck, Antibody Engineering: A Practical Guide, W.H. Freeman and Company, New York (1992).
Burtnick et al., "The isolation and characterization of the tropomyosin binding component (TN-T) of bovine cardiac troponin," Canadian Journal of Biochemistry 54(5):546-553 (1976).
Byers and Kay, "Hydrodynamic Properties of Bovine Cardiac Troponin-I and Troponin-T," J. Biol. Chem. 258:2951-2954 (1983).
Collins et al., "Early Diagnosis of Acute Myocardial Infarction with Use of a Rapid Immunochemical Assay of Creatine Kinase MB Isoenzyme," Clin. Chem. 39:1725-1728 (1993).
Adams et al., "Comparable Detection of Acute Myocardial Infarction by Creatine Kinase MB Isoenzyme and Cardiac Troponin I," Clin. Chem. 40(7):1291-1295 (1994).
Cummins et al., "Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction," American Heart Journal 113(6):1333-1344 (1987).
Cummins and Perry, "Troponin I from Human Skeletal and Cardiac Muscles," Biochem. J. 171:251-259 (1978).
Harlow and Lane, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory (1988).
Horwitz et al., "Interaction of Troponin Subunits: The Interaction Between the Inhibitory and Tropomyosin-Binding Subunits," J. Biol. Chem. 254(2):350-355 (1979).
Huse et al., "Application of a Filamentous Phage pVIII Fusion Protein System Suiutable for Efficient Production, Screening, and Mutagenesis of F(ab) Antibody Fragments," J. Immunology 149:3915-3920 (1992).
Katus et al., "Intracellular Compartmentation of Cardiac Troponin T and its Release Kinetics in Patients with Reperfused and Nonreperfused Myocardial Infarction," American Journal of Cardiology 67:1360-1367 (1991).
Katus et al., "Diagnostic Efficiency of Troponin T Measurements in Acute Myocardial Infarction," Circulation 83(3):902-912 (1991).
Leavis and Kraft, "Calcium Binding to Cardiac Troponin C.sup.1,2," Archives of Biochemistry and Biophysics 186(2):411-415 (1978).
Liao et al., "Coupling of Calcium to the Interaction of Troponin I with Troponin C from Cardiac Muscle," Biochemistry 33:12729-12734 (1994).
Liao et al., "Time-resolved tryptophan emission study of cardiac troponin I," Biophys. J. 63:986-995 (1992).
MacGeoch et al., "The human cardiac troponin I locus: assignment to chromosome 19p13.2-19q13.2," Human Genet. 88:101-104 (1991).
Metzger et al., "Skeletal troponin C reduces contractile sensitivity to acidosis in cardiac myocytes from transgenic mice," Proc. Natl. Acad. Sci. USA 90:9036-9040 (1993).
Ngai and Hodges, "Biologically Important Interactions between Synthetic Peptides of the N-terminal Region of Troponin I and Troponin C," J. Biol. Chem. 267:15715-15720 (1992).
Olah and Trewhella, "A Model Structure of the Muscle Protein Complex 4Ca.sup.2+ .multidot.Troponin C.multidot.Troponin I Derived from Small-Angle Scattering Data: Implications for Regulation," Biochemistry 33:12800-12806 (1994).
Olah et al., "Troponin I Encompasses an Extended Troponin C in the Ca.sup.2+ -Bound Complex: A Small-Angle X-ray and Neutron Scattering Study," Biochemistry 33:8233-8239 (1994).
Pearlstone and Smillie, "The interaction of rabbit skeletal muscle troponin-T fragments with troponin-I," Canadian Journal of Biochemistry and Cell Biology 63(3):212-218 (1985).
Pearlstone and Smillie, "Effects of Troponin-I Plus -C on the Binding of Troponin-T and its Fragments to .alpha.-Tropomyosin," J. Biol. Chem. 258:2534-2542 (1983).
Potter et al., "Preparation of Troponin and Its Subunits," Methods in Enzymology 85:241-263 (1982).
Pluskal et al., "Immobilon.TM. PVDF Transfer Membrane: A New Membrane Substrate For Western Blotting of Proteins," BioTechiques 4(3):272-283 (1986).
Schreier et al., "Cloning, Structural Analysis, and Expression of the Human Slow Twitch Skeletal Muscle/Cardiac Troponin C Gene," J. Biol. Chem. 265:21247-21253 (1990).
Stull and Buss, "Phosphorylation of Cardiac Troponin by Cyclic Adenosine 3':5'-Monophosphate-dependent Protein Kinase," J. Biol. Chem. 252:851-857 (1977).
Townsend et al., "Human Cardiac Troponin T: Identification of Fetal Isoforms and Assignment of the TNNT2 Locus to Chromosome 1q," Genomics 21:311-316 (1994).
Vallins et al., "Molecular cloning of human cardiac troponin I using polymerase chain reaction," FEBS Letters 270(1,2):57-61 (1990).
Wilkinson and Grand, "Comparison of amino acid sequence of troponin I from different striated muscles," Nature 271:31-35 (1978).
Wu et al., "Creatine Kinase MB Isoforms in Patients with Skeletal Muscle Injury: Ramifications for Early Detection of Acute Myocardial Infarction," Clin. Chem. 38(12:2396-2400 (1992).
Zabel et al., "Analysis of Creatine Kinase, CK-MB, Myoglobin, and Troponin T Time-Activity Curves for Early Assessment of Coronary Artery Reperfusion After Intravenous Thrombolysis," Circulation 887:1543-1550 (1993).
Zot and Potter, "Structural Aspects of Troponin-Tropomyosin Regulation of Skeletal Muscle Contraction," Ann. Rev. Biophys. Biophys, Chem. 16:535-539 (1987).
Cummins et al., "Cardiac Specific Troponin-I Radioimmunoassay in the Diagnosis of Acute Myocardial Infarction," American Heart Journal 113:1333-1344 (1987).
Bodor et. al, Clin Chem 38(11), 2203-2214, 1992.
Katus et. al, Clin Chem 38(3), 386-393, 1992.
Larve et. al Mol. Immun. 29(2), 271-278, 1992.
Larue et. al, Clin Chem, 39(6), 972-979, 1993.
Takahashi et. al Clin Chem 39(6), 1133, 1993.
Sigma Catalog, 1992.
Tietz, Text Book of Clinical Chemistry, 1986, pp. 1810, 1816.
Syska, Febs Letters, 1974, pp. 253-257, vol. 40:2.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods for the assay of troponin I and T and selection of antib does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for the assay of troponin I and T and selection of antib, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for the assay of troponin I and T and selection of antib will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1113683

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.