Method for determining the nucleotide size of DNA fragments

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

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204458, G01N 2726, G01N 27447

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active

057954550

ABSTRACT:
A method for determining the nucleotide size of DNA fragments separated by gel electrophoresis, comprising the steps of (i) measuring the migration time of each detected DNA fragment, and (ii) correlating the size of each detected DNA fragment with its migration time.

REFERENCES:
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K. Corcoran et al, "Optimal Size Colling Methods for Electphoretic Analysis Utilizing Internal Size Standards" Advances in Forensic Haemogenetics, 4, (1992) 166-168.
Richard C. Parker et al, "Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis", Proceedings of the National Academy of Science, USA, vol. 74, No. 3 (Mar. 1977) 851-855.
Brian D. Plikaytis et al, "Robust Estimation of Standard Curves for Protein Molecular Weight and Linear-Duplex DNA Base-Pair Number after Gel Electrophoresis" Analytical Biochemistry, vol. 152, No. 2 (Feb. 1986) 346-364.
Henry E. Schaffer, "Chapter 1, Determination of DNA Fragment Size from Gel Electrophoresis Mobility" Statistical analysis of DNA sequence data (1983) 1-14.
Ronald G. Duggleby et al, "A Computer Program for Determining the Size of DNA Restriction Fragments," Analytical Biochemistry, vol. 110, No. 1 (Jan. 1981) 49-55.

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