MN gene and protein

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 2431, 435 691, 4352402, 4352404, 4352523, 43525411, 4353201, 4352542, C12N 1512, C12N 1503, C12N 1504, C12N 1506

Patent

active

053876768

ABSTRACT:
A new gene--MN--and proteins/polypeptides encoded therefrom are disclosed. Recombinant nucleic acid molecules for expressing MN proteins/polypeptides and fusion proteins are provided. Expression of the MN gene is disclosed as being associated with tumorigenicity, and the invention concerns methods and compositions for detecting and/or quantitating MN antigen and/or MN-specific antibodies in vertebrate samples that are diagnostic/prognostic for neoplastic and pre-neoplastic disease. Test kits embodying the immunoassays of this invention are provided. MN-specific antibodies are disclosed that can be used diagnostically/prognostically, therapeutically, for imaging, and for affinity purification of MN proteins/polypeptides. Also provided are nucleic acid probes for the MN gene as well as test kits comprising said probes. The invention also concerns vaccines comprising MN proteins/polypeptides which are effective to immunize a vertebrate against neoplastic diseases associated with the expression of MN proteins. The invention still further concerns antisense nucleic acid sequences that can be used to inhibit MN gene expression.

REFERENCES:
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Tweedie et al. 1989. Biochemical Genet 27(1,2):17-30.
Stanbridge et al., "Specific Chromosome Loss Associated with the Expression of Tumorigenicity in Human Cell Hybrids", Somatic Cell Genetics, 7(6): 699-712 (1981).
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Zavada, "The Pseudotypic Paradox", J. Gen. Virol., 63: 15-24 (1982).
Zavada and Zavadova, "A Transmissible Antigen Detected in Two Cell Lines Derived from Human Tumours", J. Gen. Virol., 24: 327-337 (1974).
Zavada and Zavadova, "An unusual transmissible agent--MaTu", Arch. Virol., 118: 189-197 (1991).
Zavada et al., "VSV Pseudotype Produced in Cell Line derived from Human Mammary Carcinoma", Nature New Biology, 240: 124-125 (Nov. 22, 1972).
Zavada et al., "Tumorigenicity-Related Expression of MaTu Proteins in HeLa x Fibroblast Hybrids", Abstract presented at the XIX Meeting of the European Tumor Virus Group (May 1-4, 1991).

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